HPLC method for Pharmacokinetics of cis and trans isomer of cefprozil diastereomers in human plasma

Using an high-performance liquid chromatography (HPLC) system and UV detection, a simple and precise analytical procedure was developed to quantify levels of cis and trans isomer of cefprozil diastereomers in human plasma. Cefprozil was extracted from plasma samples into 10% perchloric acid. The HPLC system included an acetonitrile-0.05M monopotassium phosphate water (adjust pH to 3.05 with glacial acetic acid) (12:88, v/v), flow rate of 1 mL/min, Ultimate XB-C18 column,(5m,4.6×150mm), and UV detection at 280 nm. The regression of the spiked calibrator curves were linear from 0.02–10 μg/mL and 0.02–1 μg/mL (r =0.9951, 0.9950), respectively. The lower limits of quantification were 0.02 μg/mL, the interand intra-day precisions (RSD) were lower than 9.0%, and the extraction recoveries were all more than 90%. The cis and trans isomers of cefprozil were stable under a variety of storage and process conditions. To demonstrate utility, the pharmacokinetic parameters of cis and trans isomer of cefprozil were measured in twenty human after oral administration of 500mg cefprozil. The assay was sensitive, accurate and convenient, and can be used for the determination of cis and trans isomer of cefprozil in human plasma.